Mutagenesis of Novel Clostridial fusants for Enhanced Green Biobutanol Production from Agriculture Waste

In an earlier investigation, novel Clostridial fusants were introduced and demonstrated ability to produce biobutanol at the relatively high temperature of 45 °C. The objective present study is further improve fused strains through examining impact mutation agents on their stability, tolerance toxicity biofuel production capability. results for mutated showed enhanced resistance by better generation cells. Furthermore, (14.7–15 g/L), with a total Acetone, Biobutanol Ethanol (ABE) yield 0.6 g/g. Moreover, up 15 g/L, which equivalent ~15% increase over literature values. oxygen improved performance anaerobic fusant. general, clostridium using UV EMS leads identification stronger robust that show higher achieved yield.